| 1. | Results : a fragment of 896bp was got by rt - pcr , which contains the coding sequence of sh2 a gene
结果rt pcr扩增得到sh基因编码序列， genbank登录号为ay190323 ；连接到pcdna3 |
| 2. | Rt / pcr analysis showed that ams gene expresses in leaves , stems and flowers . the expression was n ' t detected in roots
Rt pcr分析表明ams基因在叶片、茎和花中表达，而在根中没有表达。 |
| 3. | Rt - pcr combined southern hybridization analysis show that hap2 is expressed in leaves , tepals , and tepals , stamens of floral buds
Rt pcr结合southern杂交分析表明， hapz在叶、花被片、再生花芽的花被片和雄蕊中均表达。 |
| 4. | Methods ; rt - pcr method was used to amplify the coding sequence of sh2a gene . eukaryotic recombined expression vector , pcdnas . 1 - sh2a was constructed and then transfected bel7402 cell and cos
细胞激酶活性测定相关试剂二、实验方法通过rt pcr方法扩增shzacdna编码序列，构建真核重组表达载体pcdna3 |
| 5. | And it is correlated with the lymph node status and tumor matastasis . we found the expression of vegf gene is higher in lung cancer tissue than in adjacent tissue by quantitative rt - pcr method
同时，应用荧光定量rt pcr方法检测了7例肺癌组织及相应癌旁组织中vegf的表达，发现肿瘤组织中vegf的表达水平高于癌旁组织。 |
| 6. | Mrna expression of the key elements of l - ras agt and ace mrna were expressed in myocardium , basilar arterial , carotid arterial , femoral arterial and abdominal aortic tissues as shown by rt - pcr
心血管组织l ras主要成份的mrna表达rt pcr分析结果表明，大鼠心肌、基底动脉、颈总动脉、腹主动脉和股动脉组织均有agt和ace的inrna表达。 |
| 7. | Human granulosa cells isolated from follicular fluid of 16 patients received ivf or icsi were cultured in tcm199 medium . granulosa cells were examined to detect fshr by immunobiochemistry assay and rt - pcr . 3
对16例接受体外受精患者卵泡液中分离的卵巢颗粒细胞进行体外培养，利用免疫组化法和rt pcr法检测颗粒细胞的fshr的蛋白表达和mrna的表达，鉴定体外培养颗粒细胞的纯度； 3 |
| 8. | Rt pcr showed egfr and egf gene activity were upregulated at the time of blastocyst invasion and adhension , though this upregulation was not directly reflected in an increase in bioactivity egf receptor . at meanwhile , the research of cultured endometrium cell had
本研究有助于进一步探求与子宫内膜接受性密切相关的因子在植入过程中基因表达与调控的信号转导作用，为进一步研究植入的分子机制提供理论和实践基础。 |
| 9. | Collect three groups culture supernatant for elisa experiment , rt - pcr and elisa results indicated that both transcription and translation of two cytokine genes can be enhanced obviously . rt - pcr results showed that the transcription level of il - 1 and tnf - a genes of oligochitosan contrast group were as 2
Rt pcr结果表明两种细胞因子基因转录水平明显提高，加糖对照组il一互p和tnf a基因转录水平分别为空白对照组的2 |
| 10. | Two partially overlapping cdna fragments which were cloned from o . violaceus using the rt - pcr , 3 ' race ( rapid amplification of cdna ends ) and 5 ' race technique was assembled a 1758bp full cdna sequence of epsp synthase ( accession number in genbank : af440389 )
采用rt pcr技术与3 ’ race和5 ’ race的结合，以诸葛菜的总rna为反转录的模板，克隆、拼结出了诸葛菜的epsp合成酶的全长。 dna序列（该段cdan的genbank的登录号为： af440389 ） 。 |
